Phospho flow antibodies

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August undefined, 2024

WebPhospho-SMC1A-S957 Polyclonal Antibody WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: SMAD2, SMAD3; MADH2, MADH3; MAD homolog 2, MAD homolog 3 Reactivity: Human (QC Testing), Mouse (Tested in Development), Rat (Predicted) Isotype: Mouse IgG1, κ Immunogen: Phosphorylated Human Smad2 Peptide

Phosphospecific Antibody - an overview ScienceDirect Topics

WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341. WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: Akt1, Akt2, Akt3, PKBα, PKBβ, PKBγ, RAC-PKα, RAC-PKβ, RAC-PKγ, STK-2 Reactivity: Human (QC Testing), Mouse (Tested in Development) Isotype: Mouse BALB/c IgG1, κ Immunogen: Phosphorylated Human Akt1 (pS473) Peptide speed twin tail tidy

BD™ Phosflow Protocol for Human PBMCs - BD Biosciences

WebPhospho-flow has been applied to numerous areas of biology, including antigenic stimulation and microbial challenge, high-throughput and high-content drug discovery, … WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody. WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization, Foxp3/Transcription Factor Buffer, and IC Fixation Buffer/Methanol. The recommended buffer system(s) will be noted on the … Find manuals and technical documents, get account and ordering help, browse … Flow Cytometry Support Center—Find technical support recommendations for … The Foxp3 Transcription Factor Staining Buffer Set has been formulated and … speed twin usata

Phospho-Bad (Ser136) (5D8) Mouse mAb Cell Signaling …

PE anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody - BioLegend

WebAntibody Details Format Details Citations & References Brand: BD Phosflow™ Alternative Name: SMAD2, SMAD3; MADH2, MADH3; MAD homolog 2, MAD homolog 3 Reactivity: … WebPhospho-FLT3 (Tyr591) Antibody detects endogenous FLT3 only when phosphorylated at tyrosine 591. The antibody does not cross-react with other FLT family members. It may cross-react with some tyrosine phosphorylated proteins. Species Reactivity: Human, Mouse Source / Purification speed twitch channelWebPhospho-S6 (Ser235, Ser236) Antibody (12-9007-42) in Flow. Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human phospho-S6 (S235/S236) PE, using the Intracellular Fixation and ... speed twin exhaust

"WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material. " - Phospho flow antibodies

Phospho flow antibodies

How To Do Phospho-Flow Cytometry - Cheeky Scientist

WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. WebThis Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is …

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WebAliquot an optimal concentration of phosphospecific fluorochrome-conjugated antibody to each tube. Incubate at room temperature for 30 minutes in the dark. Wash once with 2 mL … WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 …

WebPhosphoserine Antibodies Antibodies that detect Phosphoserine can be used in several scientific applications, including Western Blot, Immunohistochemistry, ELISA, Immunoprecipitation and Dot blot. Our Phosphoserine monoclonal and polyclonal antibodies are developed in Mouse and Rabbit. Find the Phosphoserine antibody that fits … WebPhospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP ® Rabbit mAb detects endogenous levels of Aurora A/B/C only when phosphorylated at either Thr288, Thr232 or Thr198 respectively. Species Reactivity: Human, Mouse, Rat Source / Purification

WebAnti-Phosphoserine antibody See all Phosphoserine primary antibodies Description Rabbit polyclonal to Phosphoserine Host species Rabbit Specificity Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human.

WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship.

WebAnti-RhoA (phospho S188) antibody See all RhoA primary antibodies Description Rabbit polyclonal to RhoA (phospho S188) Host species Rabbit Specificity Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. Tested applications Suitable for: WB more details Species reactivity Reacts with: Human speed twitch heightWebJan 1, 2012 · Antibodies: 5. Anti-human and mouse phospho-STAT3 (pY705) mouse antibody conjugated to phycoerythrin (PE) (BD/Phosflow, catalogue number: 612569). 6. Anti-human and mouse phospho-STAT5a,b (pY694) mouse antibody conjugated to Alexa Fluor ® 488 (BD/Phosflow, catalogue number: 612598). 7. speed type alphabetWebPolyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Cited in 265 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously … speed twitch streamerWebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728. speed type abcs speed twoWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl … speed type onlineWebPrimary Anti-ULK1 (phospho S556) antibody (ab203207) Primary Anti-AMPK gamma 1 antibody [Y308] (ab32508) Primary Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) View more … speed type