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WebLive or killed bacteria (gram-negative or gram-positive) can be stained with 12-15 ug/mL Hoechst or DAPI in PBS or 150 mM NaCl for 30 minutes at room temperature. Dead cells tend to stain more brightly than live cells. In S. cerevisiae, DAPI and Hoechst preferentially stain dead cells with nuclear and cytoplasmic localization. WebMay 17, 2024 · Follow the tips below to pinpoint and repair the cause of the stains, then clean, prime, and paint over the water stains to restore the blemish-free finish of your ceiling. STEP 1: Identify and ... danby upright freezers canada
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