WebRandom fragmented DNA was denaturated at 95°C for 10 min and snapped-cooled on ice for at least 5 min. 4 µg of DNA was immunoprecipitated as described previously (Chavez et al, Genome Res 2010)) using 50 µl Dynabeads (M-280 sheep anti-mouse IgG, Dynal Biotech) Grimm et al,. WebDynabeads M-280 Sheep Anti-Mouse IgG are 2.8 µm superparamagnetic beads with affinity-purified polyclonal sheep anti-mouse IgG covalently bound to the bead surface. … Dynabeads M-280 Sheep Anti-Mouse IgG are 2.8 µm superparamagnetic beads … Technical Support - Dynabeads™ M-280 Sheep Anti-Mouse IgG - Thermo Fisher … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes

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WebF , IP assay of the exosomal iodixanol gradient fractions with CD63-coated magnetic beads or unrelated Dynabeads M-280 Sheep Anti–Rabbit IgG as control. The input, unbound supernatants, and immunoprecipitated targets from the fractions were analyzed by Western blot, detecting Alix as exosomal marker and LHBs and HBcAg as HBV components. WebThis depends on the nature of the specific ligand to be immobilized and the desired downstream application. The most frequently used surface activated Dynabeads magnetic beads for philips bodygroom plus series 5000 bg5020/15

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